{"id":1861,"date":"2023-05-26T14:50:28","date_gmt":"2023-05-26T14:50:28","guid":{"rendered":"https:\/\/www.biologiaevolutiva.org\/gonzalez_lab\/?p=1861"},"modified":"2023-05-26T14:50:28","modified_gmt":"2023-05-26T14:50:28","slug":"two-step-crispr-cas9-protocol","status":"publish","type":"post","link":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/two-step-crispr-cas9-protocol\/","title":{"rendered":"Two-step CRISPR-Cas9 protocol"},"content":{"rendered":"<p style=\"font-weight: 400;\">The external protocols used in our Two-step CRISPR-Cas9 protocol for transposable element deletion in <em>D. melanogaster<\/em> natural populations can be found <a href=\"https:\/\/drive.google.com\/file\/d\/1t3VVhwKLFnn3XjwJElQ3z0pjyaFXQlWG\/view?usp=sharing\" target=\"_blank\" rel=\"noopener\">here<\/a>.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The external protocols used in our Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations can be &hellip; <a href=\"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/two-step-crispr-cas9-protocol\/\">Continue Reading<\/a><\/p>\n","protected":false},"author":4,"featured_media":1858,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-1861","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-resources"],"acf":[],"_links":{"self":[{"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/posts\/1861","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/comments?post=1861"}],"version-history":[{"count":1,"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/posts\/1861\/revisions"}],"predecessor-version":[{"id":1862,"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/posts\/1861\/revisions\/1862"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/media\/1858"}],"wp:attachment":[{"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/media?parent=1861"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/categories?post=1861"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/melanogaster.ibb-csic.info\/gonzalez_lab\/wp-json\/wp\/v2\/tags?post=1861"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}